Production and purification of TGFb-1 in CHO-Cells
نویسندگان
چکیده
Introduction The development of chemically well defined media is a demanding task in order to create the optimal conditions for an in vitro stem cell (SC) proliferation and differentiation system. Signals that govern SC differentiation into multiple mature cell types are provided by growth factors. TGFb-1 regulates a number of biological processes, including cell differentiation and proliferation, embryonic development, apoptosis and immune responses, together with cell surface receptors and signal transduction molecules within the cell. The whole signal transduction pathway leads to the specific production of distinct proteins. In this work, TGFb-1 fragment (A280 – S391) is produced using a tailor-made CHO cell line (CHO) and purified.
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